Journal: Cancer Medicine

Article Title: Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross‐Species Translational Pathway

doi: 10.1002/cam4.71775

Figure Lengend Snippet: Overall workflow of the study. Targeted* genes are ITGAM, PECAM1, CCL5, STAT1, STAT2, and CD44.

Article Snippet: The quality and quantity of RNA were determined by Nanodrop One (Thermofisher); all isolated RNA samples had A260/A280 ratios of 1.8–2.0. mRNA levels of ITGAM, PECAM, CCL5, CXCL10, STAT1, STAT2, and GZMB were determined by RT‐qPCR, relative to the housekeeping gene GAPDH, using the iTaq Universal One‐Step RT‐qPCR Kit (Bio‐Rad) and gene‐specific primers (Thermofisher; assay IDs: ITGAM Hs00167304_m1, PECAM1 Hs01065279_m1, CCL5 Hs00982282_m1, CXCL10 Hs00171042_m1, STAT1 Hs01013996_m1, STAT2 Hs01013115_g1, GZMB Hs00188051_m1).

Techniques:

Journal: Cancer Medicine

Article Title: Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross‐Species Translational Pathway

doi: 10.1002/cam4.71775

Figure Lengend Snippet: (A–F): Targeted gene (ITGAM, PECAM1, CCL5, STAT1, STAT2, and CD44) expression plots across different samples showing comparatively higher expression levels in metabolically disordered (obese) mice and humans compared to healthy individuals. Plots with p < 0.05 are only considered. Overlapping jittered points reflect individual sample variability and were retained to preserve consistent visualisation across datasets.

Article Snippet: The quality and quantity of RNA were determined by Nanodrop One (Thermofisher); all isolated RNA samples had A260/A280 ratios of 1.8–2.0. mRNA levels of ITGAM, PECAM, CCL5, CXCL10, STAT1, STAT2, and GZMB were determined by RT‐qPCR, relative to the housekeeping gene GAPDH, using the iTaq Universal One‐Step RT‐qPCR Kit (Bio‐Rad) and gene‐specific primers (Thermofisher; assay IDs: ITGAM Hs00167304_m1, PECAM1 Hs01065279_m1, CCL5 Hs00982282_m1, CXCL10 Hs00171042_m1, STAT1 Hs01013996_m1, STAT2 Hs01013115_g1, GZMB Hs00188051_m1).

Techniques: Expressing, Metabolic Labelling

Journal: Cancer Medicine

Article Title: Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross‐Species Translational Pathway

doi: 10.1002/cam4.71775

Figure Lengend Snippet: Violin plots showing the expression levels of genes of interest (ITGAM, PECAM1, CCL5, STAT1, STAT2, and CD44) across different clusters in the human pancreatic tissue, (A) non‐cancerous (control) and (B) cancerous. STAT1, STAT2, and CD44 have shown quite consistent expressions across all clusters. However, genes ITGAM, PECAM1, and CCL5 are highly expressed in clusters 8, 9, and 0 in cancerous compared to noncancerous. Identity signifies Cluster identities on the horizontal axis. Dataset used: GSE212966 .

Article Snippet: The quality and quantity of RNA were determined by Nanodrop One (Thermofisher); all isolated RNA samples had A260/A280 ratios of 1.8–2.0. mRNA levels of ITGAM, PECAM, CCL5, CXCL10, STAT1, STAT2, and GZMB were determined by RT‐qPCR, relative to the housekeeping gene GAPDH, using the iTaq Universal One‐Step RT‐qPCR Kit (Bio‐Rad) and gene‐specific primers (Thermofisher; assay IDs: ITGAM Hs00167304_m1, PECAM1 Hs01065279_m1, CCL5 Hs00982282_m1, CXCL10 Hs00171042_m1, STAT1 Hs01013996_m1, STAT2 Hs01013115_g1, GZMB Hs00188051_m1).

Techniques: Expressing, Control

Journal: Cancer Medicine

Article Title: Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross‐Species Translational Pathway

doi: 10.1002/cam4.71775

Figure Lengend Snippet: Synovial tissue gene expression profiles in healthy controls and osteoarthritis (OA) patients. Box and whisker plots display ΔCt values (y‐axis; normalised to GAPDH) for (A) ITGAM, (B) PECAM, (C) CCL5, (D) CXCL10, (E) STAT1, (F) STAT2, and (G) GZMB, quantified by qPCR. The x‐axis compares groups: Healthy Controls (synovium from neck‐of‐femur fracture patients), OA Normal‐Weight, and OA Obese. Lower ΔCt reflects higher gene expression. Whiskers span the full data range, boxes represent the IQR (25th–75th percentiles), and the central line marks the mean. Significance thresholds: “*” p < 0.05, “**” p < 0.01, “***” p < 0.001 (One‐way ANOVA with Tukey post hoc test for normally distributed data; Kruskal–Wallis with Dunn's post hoc test for not‐normally distributed data).

Article Snippet: The quality and quantity of RNA were determined by Nanodrop One (Thermofisher); all isolated RNA samples had A260/A280 ratios of 1.8–2.0. mRNA levels of ITGAM, PECAM, CCL5, CXCL10, STAT1, STAT2, and GZMB were determined by RT‐qPCR, relative to the housekeeping gene GAPDH, using the iTaq Universal One‐Step RT‐qPCR Kit (Bio‐Rad) and gene‐specific primers (Thermofisher; assay IDs: ITGAM Hs00167304_m1, PECAM1 Hs01065279_m1, CCL5 Hs00982282_m1, CXCL10 Hs00171042_m1, STAT1 Hs01013996_m1, STAT2 Hs01013115_g1, GZMB Hs00188051_m1).

Techniques: Gene Expression, Whisker Assay

Journal: Cancer Medicine

Article Title: Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross‐Species Translational Pathway

doi: 10.1002/cam4.71775

Figure Lengend Snippet: Adipose tissue gene expression profiles in osteoarthritis (OA) patients stratified by BMI. Box‐and‐whisker plots display ΔCt values (y‐axis; normalised to GAPDH) for qPCR‐measured genes: (A) ITGAM, (B) PECAM, (C) CCL5, (D) CXCL10, (E) STAT1, (F) STAT2, and (G) GZMB. The x‐axis compares the OA Normal‐Weight and OA Obese groups. Lower ΔCt values indicate higher gene expression. Whiskers show the full data range, boxes span the IQR (25th–75th percentiles), and the central line denotes the median. Significance thresholds: p < 0.05, p < 0.01, p < 0.001 ( t ‐tests or Mann–Whitney tests if not normally distributed).

Article Snippet: The quality and quantity of RNA were determined by Nanodrop One (Thermofisher); all isolated RNA samples had A260/A280 ratios of 1.8–2.0. mRNA levels of ITGAM, PECAM, CCL5, CXCL10, STAT1, STAT2, and GZMB were determined by RT‐qPCR, relative to the housekeeping gene GAPDH, using the iTaq Universal One‐Step RT‐qPCR Kit (Bio‐Rad) and gene‐specific primers (Thermofisher; assay IDs: ITGAM Hs00167304_m1, PECAM1 Hs01065279_m1, CCL5 Hs00982282_m1, CXCL10 Hs00171042_m1, STAT1 Hs01013996_m1, STAT2 Hs01013115_g1, GZMB Hs00188051_m1).

Techniques: Gene Expression, Whisker Assay, MANN-WHITNEY